Journal: bioRxiv

Article Title: LSD1 inhibitors induce neuronal differentiation of Merkel cell carcinoma by disrupting the LSD1-CoREST complex and activating TGFβ signaling

doi: 10.1101/2020.04.14.041657

Figure Lengend Snippet: a , Bulk mouse skin labeled with IgM-PE mouse isotype control. b , Bulk mouse skin labeled with NCAM-PE. DAPI - cells were sorted. c , Merkel cells labeled with IgM-PE mouse isotype control. d , Merkel cells labeled with NCAM-PE. DAPI - /NCAM + cells were sorted. Each plot shows the subpopulation gated for in the preceding plot to the left.

Article Snippet: Merkel cells were stained with anti-PSA-NCAM-PE antibody (Miltenyi Biotec, Cat. 130-117-394) or with mouse IgM-PE isotype control (Miltenyi Biotec, Cat. 130-120-070) according to manufacturer’s instructions, for 10 min at 4°C.

Techniques: Labeling, Control

Journal: Glycobiology

Article Title: Site-specific immobilization of the endosialidase reveals QSOX2 is a novel polysialylated protein

doi: 10.1093/glycob/cwae026

Figure Lengend Snippet: Proteomic analysis of polysialylated proteins from NK-92 cells. A) Immunoblot analysis of NK-92 cell lysate samples treated with PBS or with active EndoN before isolation on immobilized EndoN DM . α-polySia and α-NCAM blots show lysate (L), flowthrough (F), wash (W), and boiled beads (B). B) Volcano plot of proteins identified in samples relative to those pretreated with active EndoN. Proteins with a significant difference are labelled. The known polysialylated proteins NCAM1 and ST8Sia4 are highlighted ( n = 4 per group, assessed with student’s T-test).

Article Snippet: The antibodies used in this manuscript consisted of α-polySia mAb 735 (BioAspect BA-Ab00240-2.0), α-CD56 (R&D systems, mAB2408), α-beta-tubulin (Abcam, ab6046), streptavidin-HRP (Biolegend, 405210), goat α-mouse IgG-HRP (R&D systems, HAF007), goat α-rabbit IgG-HRP (Abcam, ab6721), and α-QSOX2 (Abcam, ab191168 and ab121376).

Techniques: Western Blot, Isolation